ALDH3A2 Knockout cell line (HEK293)

ALDH3A2 Knockout cell line (HEK293) Order

Catalog#

YKO-XN20822

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ALDH3A2 Knockout cell line (HEK293)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:6

Species

Human

Gene Name

ALDH3A2

Gene ID

224

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 1 (based on transcript ALDH3A2-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ALDH3A2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ALDH3A2 gene knockout ALDH3A2 Knockout cell line (HEK293) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ALDH3A2 Knockout cell line (HEK293)

Catalog#

YKO-XN20822

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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ALDH3A2 Knockout cell line (HEK293)

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